陳文龍教授--應用化學系暨研究所

陳文龍教授教授近照

一、學歷：

Ph.D. Georgia Institute of Technology (1992)

二、經歷：

三、專業領域：

Analytical Chemistry, Biophysical Chemistry

四、連絡方式（ Tel or e-mail)：

Tel: 886-5-271-7965

e-mail: wlchen@mail.ncyu.edu.tw　　

五、研究方向：

We are interested in the development of Raman Techniques (Dispersive Raman, FT-Raman, and Surface Enhanced Raman Scattering) to explore bioactive molecules of natural products and to characterize molecular structure of biomacromolecules. Raman spectroscopy is well known for its capability of providing rich molecular information. However, the application of Raman technique in studying natural pigments is usually hampered by the strong fluorescence signals arising from the intrinsic chromophores/fluorphors of natural pigments in plant. To overcome this problem, the development of Fourier transform Raman spectroscopy (FT-Raman) excited at near-infrared wavelength has demonstrated tremendous breakthrough. Although FT-Raman has made much progress in relaxing fluorescence interference and also has exhibited much spectroscopic superiority such as simple, direct, and free from photo bleaching due to the advantages of near infrared excitation, and the high throughput and multiplex characteristics of Michelson interferometer, it remains suffering from the shortcoming of low sensitivity, particularly for the solutions at low concentration. Therefore, to develop a high sensitive Raman technique to characterize nature pigments remains an important issue. With the aid of nano technique, we are developing surface-enhanced Raman scattering (SERS) to be an excellent ultra sensitive tool in probing trace molecules of natural pigments such as catechins and anthocyanins, of which Raman signals are usually overwhelmed by the strong fluorescence arising from their intrinsic chromophores/fluorphors. Research projects for this part include: (1) Separation, purification, and identification of bioactive compounds from Yam (2) Raman spectroscopic study of natural pigments such as catechin and anthocyanin (3) Developing ultrasensitive Raman detection system.

The second part of our research goal is to characterize protein structure by Raman spectroscopy. We have worked on eye lenses and lens proteins for years. Understanding the factors of various protein perturbations and to prevent lens from oxidative insults will lessen cataract formation that is a result of protein aggregates and remains an unsolved problem in human being. There remains no efficient method to diagnose cataract formation at its early stage, as a noninvasive tool Raman spectroscopy sheds some light on probing cataract at its early stage. The ongoing research includes purification of lens crystallins from eye lenses and characterization of their structures by Raman spectroscopy.

六、期刊著作：校務系統研究成果

1. W. Chen, I. M. Weung, and C. Y. Tseng (2003) Antioxidative and antimutagenic activities of healthy herbal drinks from Chinese medicinal herbs, Amer. J. Chin. Med. 31, 4, 1-10. SCI

2. C. L. Shiu, W. Chen, I. M. Weng, and C. Y. Tseng (2003) Chemical composition, physical properties and antioxidant activities of yam flours as affected by different drying methods., Food Chem., 83. 85-92. SCI

3. Y. M. Weng, R. H. Weng, C. Y. Tzeng, and W. Chen* (2003) Structural Analysis of Triacylglycerols and Edible Oils by Near-Infrared Fourier Transform Raman Spectroscopy. Applied Spectroscopy, 57, 4, 413-418. SCI

4. C. H. Huang, R.J. Chang, S. L. Huang, and W. Chen (2003) Dietary Vitamin supplementation affects tissue lipid peroxidation of hybrid tilapia, Oreochormis niloticus x O. aureus’. Comp. Biochem. and physiol, 134, 265-270.

5. S. H. Shih, Y. M. Weng, S. L. Chen, S. L. Huang, C. H. Huang, and W. Chen* (2003) FT-Raman Spectroscopic Investigation of Lens Proteins of Tilapia Treated with Dietary Vitamin E. Arch. Biochem. & Biophys., 420, 79-86. SCI

6. C. L. Hsu, S. L. Hurang, W. Chen, and Y. M. Weng, and C. Y. Tseng (2004) Qualities and antioxidant properties of bread as affected by the incorporation of Yam flour in the formulation. Internal. J. Food Sci. & Tech., 39,231-238. SCI

7. L. Y. Kuo, G. Y. Huang, S. L. Yang, Y. W. Hua, W. Chen, and L. L. Lin (2004) Inactivation of Bacillus stearothermophilus leucine aminopectidase II by hydrogen peroxide and site-directed mutagenesis of methionine residues on the enzyme. Protein J., 23, 295-302. SCI

8. M. C. Chi, W. M. Chou, C. H. Wang, W. Chen, W. H. Hsu, and L. L. Lin (2004) Generating oxidation-resistant variants of Bacillus kaustophilus leucine aminopeptidase by substitution of the critical methionine residues with leucine. J.Gen. & Appl..Microbiol. 1-8. SCI

9. Y. H. Liao, C. H. Wong, C. Y. Tseng, H. L. Chen, R. L Lin, and W. Chen* (2004) Compositional and Comformational Analysis of Yam Proteins by Near Infrared Fourier Transform Raman Spectroscopy. J. Agric. & Food Chem. 52, 8190-8196. SCI

10. B. L Hsu, I. M. Weng, Y. H. Liao, and W. Chen* (2005) Structural investigation of edible zein films/coatings and directly determining their thickness by FT-Raman spectroscopy. J. Agric. & Food Chem. 53, 5089-5095 SCI

11. Y. H. Liao, C. Y. Tseng, and W. Chen* (2006) Structural characterization of dioscorin, the major tuber protein of yams, by near infrared Raman spectroscopy. J. Physics: conf. ser., 28, 119-122.

12. R. H. Weng, I. M. Weng,. C H. Wong, K. L. Ku, and W. Chen* (2006) Authentication of Camellia oleifera Abel Oil by Near Infrared Fourier Transform Raman Spectroscopy. J. Chin. Chem. Soc. 53, 1-7. SCI

13. P. Y. Chou, C. H. Hong, W. Chen, Y. J. Li, Y. S. Chen, R. Y.-Y. Chiou (2006) A glass distilling collector applied for HCN recovery from submerged-cultivated broth and fruiting body of Pleurotus eryngii for identification and quantification. J. Agri. & Food Chem. 54, 1551-1556. SCI

14. M. Liang, V. Chen, H. L. Chen, and W. Chen*(2006) A simple and direct isolation of whey components from raw milk by gel filtration chromatography and structural characterization by Fourier transform Raman spectroscopy. Talanta 69, 1269-1277. SCI

15. H. L. Yang, R. S. Chen, W. Chen, L. L. Lin (2006) Identification of glutamate residues important for catalytic activity of Bacillus stearothermophilus leucine aminopeptidase II. Antonie van Leeuwenhoek Inter. J. Gen. & Mole. Microbiol. 90,195-199 SCI

16. C. T. Liu, P. T. Chuang, C. Y. Wu, Y. M. Weng, W. Chen, and C. Y. Tseng (2006) Antioxidative and in vitro hepatoprotective activity of bupleurum kaoi leaf infusion. Phytother. Res. (in press) SCI

17. T. F. Lien, W. Chen, Y. L. Hsu, H. L. Chen, R. Y.-Y. Chiou (2006) Influence of Soy Aglycone Isoflavones on Bone-related Traits and Lens Protein Characteristics of Ovariectomized Rats and Bioactivity Performance of Osteoprogenitor Cells, J. Agri. & Food Chem. (Accepted) SCI

18. Wenlung Chen陳文龍 (2009 ) Structural Studies of the Complex Between Akt-in and the Akt2-PH Domain Suggest that the Peptide Acts as an Allosteric Inhibitor of the Akt Kinase. The Open Spectrosco. J. 3,65-76

19. A. K. H. Chen, R. Y. Y. Lin, E. Z. J. Hsieh, P. H. Tu, R. P. Y. Chen, T. Y. Liao, W. Chen, C.--H. Wa (2010) Induction of Amyloid Fibrils by the C- Terminal Fragments of TDP-43 in Amyotrophic Lateral Sclerosis. J. Am. Chem. Soc. 132,1186-1187

20. P.-C. Shih, G.-C. Li, K.-J. Yang, W. Chen, D.-L. Tzou* (2011) Conformational analysis of steroid hormone molecules in the lipid environment - A solid-state NMR approach. Steroids 76,558-562

21 .G.-C. Li, D-R. Wang, W. Chen, D.-L. M. Tzou* (2012) Solid-state NMR analysis of steroidal conformation of 17α- and 17β-estradiol in the absence and presence of lipid environment. Steroids 77,185-189

22. 陳文G. C.-H Liu, B. P.-W Chen, N. T.-J. Ye, C.-H. Wang, W. Chen, H.-M. Lee, S. I. Chan, J. J.-T. Huan (2013) Delineating the membrane-disrupting and seeding properties of the TDP-43 amyloidogenic core. Chem. Comm. 49, 11212-11214

23. C.-H. Wang, M.-J. Chen, C.-Y. Tseng, K.-M. Wei, W. Chen* (2013) Structural characterization of functional compositions isolate from Dioscorea Purpurea (Cultivar of Ming-Chien) by Raman spectroscopy. Natural Product and Chemistry Research 1,110

24. D-R. Wang, J-C. Hsiao,C-H. Wong, G-C Li, S-C. Lin, S. S-F. Yu, W. Chen, W. Chang and D-L. M. Tzou* (2014) Vaccinia Viral Protein A27 is Anchored to Interaction with Viral Membrane Protein A17 the Viral Membrane via a Cooperative. Journal of Biological Chemistry. 289, 6639-6655

25. C-S. Sun, C. Y- H. Wang, P-W. Chen, R-Y. He, C-H. Liu, C-H. Wang, W. Chen, Y. Chern, J. J.T. Huang (2014) The influence of pathological mutations and proline substitutions in TDP-43 Glycine-rich peptides on its amyloid properties and cellular toxicity. PLOSone 9, e103644

26. 陳文龍(2015) Conformational switch of polyglutamine-expanded huntingtin into benign aggregates leads to neuroprotective effect. Scientific reports 5, 14992

27. J. S. Jin, L. F. Lin, J. C. Chen, C. C. Huange, J. H. Sheu, W. Chen, T. Y. Tsao, and C. W. Hsu (2015) Increased cyclin T1 expression as a favorable prognostic factor intreating gastric adenocarcinoma. Oncology Letters 10, 3712-3718

28. C. H. Wang, C.C. Huang, L.L. Lin, and W. Chen* (2016) The Effect of Disulfide Bonds on Protein Folding, Unfolding, and Misfolding Investigated by FT-Raman Spectroscopy. Journal of Raman Spectroscopy 47,00

29. LIEN. F. Lin , JONG. S. Jin , J. C. Chen , C. C. Huang , J. H. Sheu , W. Chen, and C. W. Hsu(2016) Positive cyclin T expression as a favorable prognostic factor in treating gastric gastrointestinal stromal tumors. MOLECULAR AND CLINICAL ONCOLOGY 10, 3892

30. C. W. Hsu, C. C. Huang, J. H. Sheu, C. W Lin, L. F. Lin, J. S Jin, L. K. Chau, W. Chen*(2016) Differentiating gastrointestinal stromal tumors from gastric adenocarcinomas and normal mucosae using confocal Raman microspectroscopy. Journal of Biomedical Optics 21,00

31. C. W. Hsu, C. C. Huang, J. H. Sheu, C. W. Lin, L. F. Lin, J. S. Jan, L. A. Chau, W. Chen*(2016) Novel Method for Differentiating Histological Types of Gastric Adenocarcinoma by Using Confocal Raman Microspectroscopy. PLoS One 0,1-12

32. C. L. Huang, W. Chen, M. S. Tsai*, C. H. Ciou, H. Y. Hsu, and . C. C. Ting*(2017) Reversibly switching surface-enhanced Raman scatering based on colloidal electrophoresis. Optics Express 25, 32436-32442

33. C. C. Huang and W. Chen*(2017) Raman spectroscopic analysis of cataract lens: A compendious rview. Applied Spectroscopy Review 0,1-14

34. Chih-Hsien Wang, Chia-Chi Huang, and Wenlung Chen*(2017) Simultaneous Separation of Taxon-Specific Crystallins from Mule Duck and Characterization of Their Enzymatic Activities and Structures.Journal of Chromatography B 1053,34-41

35. C.-C. Huang* and W. Chen*(2018) A SERS method with attomolar sensitivity: a case study with the flavonoid catechin. Microchimica Acta 1,1-8

36. C.L Huang, C.C. Huang* and W. Chen*(2018) A report from the 5th international Taiwan symposium on Raan spectroscopy and Taiwan association of Raman spectroscopy summer camp.Journal of Raman Spectroscopy 10,1-6

七、研討會論文

1. R. H. Weng, I. Weng and W. Chen* (2002) Characterization of edible oil by near IR Fourier transform Raman spectroscopy. XIIth International Conference on Raman Spectroscopy. 817-818.

2. 翁汝卉, 翁義銘, 陳文龍* (2002) 傳立葉轉換拉曼光譜探討食用油脂之特性,中國化學會91年年會論文手冊 AN-13.

3. 徐寶蓮, 翁義銘, 陳文龍* (2002) 拉曼光譜偵測L-半胱胺酸之穩定性, 中國化學會91年年會論文手冊 BC-01.

4. 莊攸禎, 鄧佳容, 陳文龍, 蔣宗哲, 蔡滄朝, 曾慶瀛 (2002) 中藥酒製程規格化及其貯存安定性之研究, 中華民國食品科學技術學會卅二次會員大會手冊 CO-10.

5. 廖育秀, 陳秀玲, 曾慶瀛, 陳文龍* (2003) 山藥蛋白質的純化及拉曼光譜結構分析, 中國農業化學會第四十一次會員大會C-58.

6. S. H. Shih, Y. M. Weng, and W. Chen* (2003) Study on the vitamin E protection of lens proteins against lipid peroxidation by FT-Raman spectroscopy. Fourth East Asian Biophysics Symposium. p.100.

7. 王智賢, 陳文龍* (2003) 山藥花青素之純化及光譜分析, 中國化學會92年年會論文手冊 AN-05.

8. 張高逢, 王智賢, 陳文龍, 何永皓 (2003) 紫田薯中花青素的分析, 中國化學會92年年會論文手冊 AN-07.

9. 陳秀玲, 王壁娟, 曾慶瀛, 陳文龍* (2003) 晶狀體中麩胱甘肽之定量分析, 中國化學會92年年會論文手冊 AN-08.

10. 翁汝卉, 翁義銘, 陳文龍* (2003) 傳立葉轉換拉曼光譜應用於食用油脂之摻假, 中國化學會92年年會論文手冊 AN-44.

11. 徐寶蓮, 施秀鶴, 翁義銘, 陳文龍* (2003) 以拉曼光譜分析半胱胺酸之氧化反應, 中國化學會92年年會論文手冊 BC-08.

12. 施秀鶴, 翁義銘, 陳文龍* (2003) 維生素E及麩胱甘肽於晶體蛋白中之抗氧化機制研究, 中國化學會92年年會論文手冊 BC-08.

13. 廖育秀, 陳秀玲, 曾慶瀛, 陳文龍* (2003) 山藥蛋白質純化及拉曼光譜結構分析(二), 中華民國食品科學技術學會卅三次會員大會手冊 AP-46.

14. 翁汝卉, 王智賢, 翁義銘, 曾慶瀛, 陳文龍* (2003) 紫色山藥粗花青素之抗氧化活性探討, 中華民國食品科學技術學會卅三次會員大會手冊 EP-09.

15. 徐寶蓮, 翁義銘, 陳文龍* (2003) 以拉曼光譜測定可食性玉米蛋白膜之厚度中華民國食品科學技術學會卅三次會員大會手冊 FP-18.

16. 楊書僕, 翁義銘, 陳文龍* (2003) 以共軛焦顯微拉曼光譜測定塑膠積層膜之組成及厚度, 中華民國食品科學技術學會卅三次會員大會手冊 FP-19.

17. 莊攸禎，曾慶瀛, 陳文龍* (2003)非傳統製備藥酒及其抗氧化特性之探討, 中華民國食品科學技術學會卅三次會員大會手冊 CO-05.

18. R. H. Weng and Wenlung Chen* (2004) Authentification of Camellia Oleifera Abel oil by near infrared Fourier transform Raman spectroscopy. XIXth International Conference on Raman Spectroscopy. XIXth International Conference on Raman Spectroscopy.

19. L.L. Lin, Y.S. Liao, and Wenlung Chen* (2004) FT-Raman spectroscopic study of yam proteins conformation. XIXth International Conference on Raman Spectroscopy. XIXth International Conference on Raman Spectroscopy.

20. Y. M. Weng, Y. C. Chuang, W. Chen, and C. Y. Tseng (2004) Ingredient extracting efficiency and functional properties of Chinese medicinal herbal wines as affected by ultrasound-assisted extraction.2004 IFT Annual Meeting and Food Expo. (Las Vegas, Nevada). 114F-20.

21. C.Y. Tseng, C. T. Liu, W. Chen, and Y. M. Weng (2004) Antioxidative properties and in vitro hepato-protective activity of Bupleurum kaoi leaf infusion. 2004 IFT Annual Meeting and Food Expo. (Las Vegas, Nevada) 33F-18.

22. 李興傑, 邱亭錡, 陳文龍* (2004) 山藥蛋白質純化及FT-Raman光譜分析, 中國化學會93年年會論文手冊 ANA-C-063.

23. 翁汝卉, 王智賢, 翁義銘, 陳文龍* (2004), 傅立葉轉換拉曼光譜鑑定苦茶油真偽, 中國化學會93年年會論文手冊 ANA-C-064.

24. 廖育秀, 曾慶瀛, 陳文龍* (2004) 傅立葉轉換拉曼光譜探討山藥蛋白質之熱凝性, 中國化學會93年年會論文手冊 ANA-C-065.

25. 黃家琪, 陳文龍*(2004) 傅立葉轉換表面增強拉曼效應於兒茶素水溶液之應用, 中國化學會93年年會論文手冊 ANA-C-066.

26. 黃家琪, 陳文龍*(2004) 表面增強拉曼效應於兒茶素之應用, 中國化學會93年年會論文手冊 ANA-C-067.

27. 楊書僕, 翁義銘, 陳文龍* (2004) 以共軛焦顯微拉曼光譜分析薄層聚合物之組成, 中國化學會93年年會論文手冊 ANB-D-264.

28. 陳秀玲, 王智賢, 曾慶瀛, 陳文龍* (2004) 鴨眼球δ-Crystallin之純化及拉曼光譜結構分析, 中國化學會93年年會論文手冊 BC-C-124.

29. 廖育秀, 陳秀玲, 林榮流, 曾慶, 陳文龍*(2004) 以傅立葉轉換拉曼光譜分析山藥蛋白質組成及構形之探討, 台灣食品科技學會D-24.

30. 陳秀玲, 王璧娟, 曾慶瀛, 陳文龍*(2004) 攝取靈芝對老鼠晶狀體中GSH含量之影響, 台灣食品科技學會D-23.

31. Y. H. Liao, H. L. Chen, C. Y. Tseng, R. L Lin, and W. Chen* (2005) Structural Characterization of Dioscorins, the Major Tuber Protein of Yams, by Near Infrared Fourier Transfer Raman Spectroscopy. 3^rd International conference on Materials for Advanced Technologies Symp. Y Pp.16.

32. R. Y.-Y. Chiou, P.-Y Chou, C.-H. Hong, W. Chen, and Y.-J. Li (2005) Recovery of HCN for Identification and Quantification from Submerged-Cultivated Broth and Fruiting Body of Pleurotus Eryingii. 2^nd International Symposium on Recent Advances in Food Analysis”, p.285.

33. Y. H. Liao, C. Y. Tseng, and W. Chen* (2005) Raman Spectroscopic Study of Dioscorins, the Major Storage protein of Yam. IFT Annual Meeting and Food Expo. (New Orleans, LA). 54E-23.

34. C. H. Wang, C. Y. Tseng, and W. Chen* (2006) Structural identification of functional compositions isolated from d. alata L. var. purpurea. IFT Annual Meeting and Food Expo. (Orlando, FL). 54E-23.

35. C. C. Huang and W. Chen* (2006) SERS of natural pigments. XX^th International Conference on Raman Spectroscopy.

36. C. H. Wang, C. Y. Tseng, and W. Chen* (2006) FT-Raman spectroscopic analysis of functional compositions isolated from d. alata L. var. purpurea. XXth International Conference on Raman Spectroscopy.

八、研究計畫：

A. 國科會

研發高靈敏拉曼光譜技術直接分析天然多酚類化合物及快速檢測微生物生長 (1/3)（主持人；94-2113-M-415-001）

以石墨爐原子吸收光譜法偵測中藥硒之基質干擾探討 (共同主持人；94-2113-M-415-011-)

研發拉曼光譜技術探討花青素之特性(2/2) (主持人；94-2113-M-415-001-)

研發拉曼光譜技術探討花青素之特性 (1/2) (主持人；93-2113-M-415-007- )

研發拉曼光譜技術探討山藥蛋白質之結構 (共同主持人；93-2313-B-415-006- )

監測小量組織培養代謝物產物二苯乙烯衍生物之液相層析質譜方法 (共同主持人；93-2113-M-415-006- )

拉曼光譜分析蛋白質中半胱胺酸之氧化還原性質 (主持人；92-2113-M-415-008- )

以拉曼光譜法定量食品表面玉米蛋白可食性膜 (共同主持人；92-2214-E-415-003- )

苦茶(Camellia oleifera Abel)油中主要抗氧化成分分析方法之開發 (共同主持人；92-2113-M-415-009- )

藥用及保健植物-山藥之研發與利用－山藥多醣體、蛋白質特性研究以及利用擠壓技術開發山藥保健食品(共同主持人；91-2317-B-415-001- )

結合HPLC,CZE及拉曼光譜技術同步偵測及定量眼睛組織中之麩胱甘太(II)( 主持人；90-2113-M-415-002- )

結合HPLC,CZE 及拉曼光譜技術同步偵測及定量眼睛組織中之麩胱甘胺太(I) (主持人；89-2113-M-415-002- )

B. 農委會

以擠壓技術製成組織化山藥素肉產品之研究（主持人；2004/01/01 至 2004/12/31）

九、其他：

十、教授課目：

高等分析化學　普通化學　實驗