李互暉 副教授 

Hu-Hui Lee  Ph.D.

專長：

動物基因及發育、小鼠基因剔除與基因轉殖、幹細胞研究、生物醫學動物模式研究，單株抗體製備

教授科目：

動物基因轉殖與細胞培養及實驗、動物發育學、動物生殖學、幹細胞培養與應用、基因與發育、生物影像技術、動物分子遺傳學、生物學及實驗、生物技術及實驗、動物繁殖與育種、科學論文寫作、專題討論

辦公室電話：05-2717768

系辦公室傳真：05-2717755

e-mail：hlee@mail.ncyu.edu.tw

學歷：

2003年6月，Doctor of Philosophy, University of Texas Houston, Houston, Texas美國德州大學休士頓健康科學中心博士

1991年6月，Master of Science, Chinese Culture University, Taipei, Taiwan文化大學生物科技研究所碩士

1983年6月，Bachelor of Science, National Chung-Hsing University, Taichung, Taiwan中興大學土壤學系學士  

經歷：

 2014，8～ present Associate Professor, National Chiayi University, Department of Bioagriculture Science, Chiayi, Taiwan. 國立嘉義大學生物農業科技學系副教授

 2007，8～2014，7 Assistant Professor, National Chiayi University, Department of Bioagriculture Science, Chiayi, Taiwan. 國立嘉義大學生物農業科技學系助理教授

 2003，10～2007，7 Postdoctoral Fellow, University of Washington, Seattle, WA, USA 

美國華盛頓大學博士後研究

 2003，6～2003，10 Postdoctoral Fellow, University of Texas, M.D. Anderson Cancer Center, Houston, TX, USA 

美國德州大學安德森癌症中心博士後研究

 1996，8～2003，6 Graduate Research Assistant, University of Texas, M.D. Anderson Cancer Center, Houston, TX, USA 美國德州大學安德森癌症中心研究助理

 1993，6～1996，6 Research Assistant, Institute of Molecular Biology, Academia Sinica, Taipei, Taiwan 

中研院分子生物研究所碩士級研究助理

 1990，3～1991，8 Senior Research Technician, Developmental Center for Biotechnology, Taipei, Taiwan 

生物技術開發中心助理研究員

 1988，6～1990，3 Supervisor of Quality Control Section, Bristol-Myers Squibb Taiwan Pharmaceutical Branch, Hsin-Chu, Taiwan 施貴寶大藥廠資深品管員

 1987，8～1988，6 Assistant Engineer, General Life Biotechnology, Hsin-Chu, Taiwan 

普生生物科技有限公司助理研究員

 1985，10～1987，7 Research Assistant, Panlab Pharmaceutical Research Institute, Taipei, Taiwan 

泛球藥理研究所研究員

論文發表

期刊論文

1. 李互暉、廖芸郁、蔡依錦 、劉奎均、陳立耿、鄭可大.(2014)以細胞模式篩選可能抑制骨質疏鬆的植株萃取物. 嘉大農林學報 11(1):63-74.

2. Hu-Hui Lee, Jing-Yu Lee & Lan-Hsin Shih. (2013) Proper Fhl1 expression as Wnt signaling is required for chondrogenesis of ATDC5 cells. Animal Cells and Systems 17 (6):413-20 (SCI)

3. Lee HH, Sung MH. (2013) The neutrophil elastase mutant affects viability and differentiation of the human monocytic THP-1 cell. Cell Biochem Funct. 31(4): 305-11. (SCI)

4. Lee JY, Chien IC, Lin WY, Wu SM, Wei BH, Lee YE, Lee HH. (2012) Fhl1 as a downstream target of Wnt signaling to promote myogenesis of C2C12 cells. Mol Cell Biochem. 365(1-2):251-62. (SCI)

5. Nelson A. Arango, Akio Kobayashi, Ying Wang1, Soazik P. Jamin1, Hu-Hui Lee, Grant D. Orvis, and Richard R. Behringer (2008). A mesenchymal perspective of Müllerian duct differentiation and regression in Amhr2-lacZ mice. Molecular Reproduction and Development 75(7): 1154 – 62 (SCI)

6. Duan Z, Person RE, Hu-Hui Lee, Huang S, Donadieu J, Badolato R, Grimes HL, Papayannopoulou T, Horwitz MS (2007). Epigenetic Regulation of Protein-Coding and MicroRNA Genes by the Gfi1-Interacting, Tumor Suppressor PRDM5. Mol Cell Biol. 27(19): 6889-6902 (SCI)

7. Hu-Hui Lee and Richard R. Behringer (2007). Conditional expression of Wnt4 during Chondrogenesis leads to dwarfism in mice. PLoS ONE 2(5): e450 (SCI)

8. Marshall S. Horwitz, Zhijun Duan, Brice Korkmaz, Hu-Hui Lee, Matthew E. Mealiffe, and Stephen J. Salipante (2007). Neutrophil elastase in cyclic and severe congenital neutropenia. Blood 109(5):1817-24 (SCI)

9. Hu-Hui Lee, Wen-Hsui Chiang, Shian-Huey Chiang, Yin-Chang Liu, Jaulang Hwang, and Sun-Yu Ng (1995). Regulation of Cyciln D1, DNA Topoisomerase I, and Proliferating Cell Nuclear Antigen Promoters During the Cell Cycle. Gene Expression. 4: 95-109. (SCI)

10. Shu-Hui Liu, Hu-Hui Lee, Jia-Jia Chen, Chiou-Fen Chuang, and Sun-Yu Ng (1994). Serum Response Element-regulated Transcription in the Cell Cycle: Possible Correlation with Microtubule Reorganization. Cell Growth & Differentiation. 5:447-455. (SCI)

研討會論文

1. Yi-Jun Shen, Lan-Hsin Shih and Hu-Hui Lee. The influence of polybrene and protamine sulphate in mesenchymal stem cell transduction by lentivirus.中華實驗動物學會第十三屆學術研討會. 2013/12

2. Lan-Hsin Shih and Hu-Hui Lee. Estrogen represses Fhl1 to stimulate bone mineralization. 中華實驗動物學會第十二屆學術研討會. 2012/12

3. Hu-Hui Lee*, Jing-Yu Lee, I-Chun Chien, Win-Yu Lin, Shao-min Wu, Bo-Huei Wei, and Yu-En Lee. Fhl1 promotes myogenesis of C2C12 in response to Wnt signaling. SDB 71st Annual Meeting July 19 - 23, 2012, Montreal, Canada, p80-81, Montreal, Canada.

4. Yun-Yu Liao and Hu-Hui Lee. Screening plant extracts and functional studies on bone mineralization. 中華實驗動物學會第十一屆學術研討會, Dec. 6-7, 2011.

5. Bo-Huei Wei and Hu-Hui Lee. Use lentivirus system to express Wnt signaling in lung tissue. 中華實驗動物學會第十一屆學術研討會, Dec. 6-7, 2011.

6. Chun Chien and Hu-Hui Lee. The cloning and expression of the lung-specific sp-c promoter for studying Wnt4 gene function in lung branching morphogenesis. 中華實驗動物學會第十一屆學術研討會, Dec. 6-7, 2011.

7. Win-Yu Lin and Hu-Hui Lee. The function of Wnt4 gene in murine lung development. 中華實驗動物學會第十一屆學術研討會, Dec. 6-7, 2011.

8. Jing-Yu Lee and Hu-Hui Lee. The canonical Wnt-inducible factor Fhl1 inhibits chondrogeneis of ATDC5 cells. WNT 2011 annual meeting, June 29-July 3, 2011. Los Angel, USA

9. Shao-min Wu and Hu-Hui Lee. The possible association of Fhl1 and estrogen signaling in bone mineralization. 轉譯科學之動物生技學術研討會, 2010,01.

10. Ming-Han Sung and Hu-Hui Lee. The effect of mutated neutrophil elastase in neutropenia. 前瞻農業科技國際研討會, 2009,08.

11. Hu-Hui Lee and Richard R. Behringer. (2002). Conditional Expression of Wnt4 During Skeleton Development.  The American Society for Matrix Biology, Houston, Texas, USA.

12. Hu-Hui Lee and Richard R. Behringer. (2002). Conditional Expression of Wnt4 During Skeleton Development. Mouse Molecular Genetics Meeting 2002, Cold Spring harbor, New York, USA.

13. Hu-Hui Lee and Richard R. Behringer. (2001). Conditional Expression of Sox9 and Sry in Mouse Ovaries. The 54th Annual Symposium on Fundamental Cancer Research – Mechanism for Cell Growth and Differentiation, Houston, Texas, USA.

14. Hu-Hui Lee and Richard R. Behringer. (2001). Conditional Gene Expression of Sox9. The 9th SCBA international Symposium Society of Chinese Bioscientists in America, Taipei, Taiwan.

15. Hu-Hui Lee and Richard R. Behringer. (2000). Differential Screen for Sex-Specific Genes in the Mouse.  The second International symposium on the Biology of vertebrate Sex Determination, Honolulu, Hawaii, USA.

16. Chao-Wei Liao, Hu-Hui Lee, Shinn-Liang Lai, and Jaulang Hwang. (1995). Establish a Protein System to Target p53 into Cells. Chinese Annual Conference of Biomedical Sciences, The Sixth SCBS international Symposium, Vancouver, B.C., Canada.

17. J. H. Huang, H. H. Lee, Y. S. Chiang, C. Y. Lan, K. C. Shen and T. H. Chang (1991). Production of Monoclonal Antibodies to Human Leucocytes Differentiation Antigens. Chinese Annual Conference of Biomedical Sciences, Taipei, Taiwan.

專書論文及技術報告：

1. Hu-Hui Lee (2002). Conditional expression of Sry, and Wnt4 by gene-targeting: studies in sexual and skeletal development. Graduation thesis

2. Hu-Hui Lee and Richard R. Behringer (2002). Conditional Expression of Wnt4 During Skeleton Development.  The American Society for Matrix Biology, Houston, Texas, USA.

3. Hu-Hui Lee and Richard R. Behringer (2002). Conditional Expression of Wnt4 During Skeleton Development.  Mouse Molecular Genetics Meeting 2002, Cold Spring harbor, New York, USA.

4. Hu-Hui Lee and Richard R. Behringer (2000). Differential Screen for Sex-Specific Genes in the Mouse.  The second International symposium on the Biology of vertebrate Sex Determination, Honolulu, Hawaii, USA.

5. Lee,H.-H. (2001). Mus musculus SRY sex determining region Y-box 9 protein (Sox9) mRNA, complete cds. Accession: AF421878, GI:16226172. NCBI GenBank. http://www.ncbi.nlm.nih.gov/entrez/

專利

1. National Patent for Production of Monoclonal Antibodies against T helper cells, 1990, TW 081103959. T輔助細胞表面抗原單株抗體製作

2. National Patent for Production of Monoclonal Antibodies against cytotoxic T cell, 1990, TW 080103126. T毒殺細胞表面抗原單株抗體製作

學術活動與榮譽

1. Excellent advisor award, National Chiayi University, 2011. 國立嘉義大學99學年度優良導師獎

2. Honorable Mention, The 7th Annual Trainee Recognition, University of Texas, M.D. Anderson Cancer Center, 2003

3. Travel award, The 9th SCBA international Symposium Society of Chinese Bioscientists, 2001

4. Bettyann Asche-Murray Ovarian Cancer Fellowship 2000

5. Research Poster Achievement Award, Genes and Development Program, University of Texas, Spring Retreat 1999

6. Champion of the Thesis Presentation in the Institute of Molecular Biology, Academia Sinica, Taiwan, 1993

7. Graduated in the first place in class (graduate school) and obtained the University Award, 1993

8. National Scholarship from the Ministry of Education, 1992

9. Chief Editor of a Scientific Journal (Soil Science) in a Student Association, 1983

10. Hsieh's Scholarship for the Outstanding Academic performance in the Student Body of the University, 1983.